Calf Thymus Composition. A Comparison of Differential Centrifugation and Chemical Fractionation Procedures
نویسندگان
چکیده
منابع مشابه
Calf Thymus Composition. A Comparison of Differential Centrifugation and Chemical Fractionation Procedures
The extraction behavior of thymus and the composition of fractions prepared from this organ has been studied. Sequential extraction methods using 0.15 M NaCl followed by water gave information with respect to the weight fraction of cytoplasmic and nuclear constituents. Lipide, nucleic acid, and electrophoretic analysis of the extracts provided additional information. A less complex electrophore...
متن کاملCalf Thymus Composition. A Comparison of Differential Centrifugation and Chemical Fractionation
The extraction behavior of thymus and the composition of fractions prepared from this organ has been studied. Sequential extraction methods using 0.15 NaC1 followed by water gave information with respect to the weight fraction of cytoplasmic and nuclear constituents. Lipide, nucleic acid, and electrophoretic analysis of the extracts provided additional information. A less complex electrophoreti...
متن کاملChromatographic fractionation of calf thymus histone.
Evidence has recently accumulated that histone preparations, particularly those derived from the nuclei of calf thymus, may contain a mixture of different basic proteins. Variations in the amino acid composition of histone fractions extracted and precipitated by different means have been demonstrated by Stedman and Stedman (2), Daly, Mirsky, and Ris (3), Eadie and Leaf (4), Hamer (5), Davison a...
متن کاملFurther studies on the fractionation of calf thymus histone.
The fractionation of calf thymus histone has been studied in the past few years by many investigators. The gross separation of this mixture into fractions which differed in physicochemical properties, or its more minute resolution into components that were presumed, in some instances, to be fairly homogeneous has been reported by the following workers: Ahlstrom (l), Stedman and Stedman (2), But...
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ژورنال
عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology
سال: 1958
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.4.6.717